The food and feeding of the brackish river prawn Macrobrachium macrobrachion from Ekole creek, Bayelsa State, Nigeria was studied from June to August 2013. Samples of the shrimp were collected fortnightly from Swali community (Station 1) and Agbura community (Station 2) at Ekole creek using a non-return valve trap locally called orkow. The sizes of specimen ranged from 3.40 cm to 10.5 cm and weighed between 0.5 g and 18.3 g with males generally larger than the females. The male ranged in size from 3.40 cm to 10.50 cm and weighed between 6 g and 18.30 g while the females ranged from 3.50 cm to 9.10 cm and weighed between 0.5 g and 10.10 g. The gut of each shrimp was dissected and examined under a high precision microscope (Model BM-100 Series XSZ-701/XSZ-107). The food items were identified with standard keys. The diet from the gut was analyzed using the frequency of occurrence and the point’s methods. It was found that the prawn had an omnivorous diet feeding on a wide variety of organisms such as phytoplankton, plants, insect parts and larvae, zooplankton and unidentifiable items. The relative importance index (RI) showed that the diet is influenced by availability as most of the species prefer phytoplankton and plant parts while a few had zooplankton and insect parts. The shrimp can be said to be omnivorous. The prawns analyzed were classified as juveniles, young adults and adults with the young adults constituting majority of its population. The males were more in number than the females. The values obtained from the length/weight relationship (LWR) of M. macrobrachion showed a positive, significant correlation between total length and weight (r = 0.94). The condition factor, K of the shrimp provides information on the physiological state of the shrimp in relation to its welfare. The condition factor, K was between 1.274 and 1.513 for both male and female specimens; while ‘K’ values for male was 1.507 and female, 1.293. This showed that the species is in good condition in Ekole Creek, Bayelsa State, Nigeria.